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Chambers 6 min read

Diagnosing temperature and humidity drift in a growth chamber

Chamber drift is slow enough to miss and consequential enough to invalidate a trial. Here is how to catch it.

Interior of a controlled environment growth chamber with seedlings on shelves under magenta and blue LED lighting

Drift is dangerous because it is gradual

A chamber that fails outright is an obvious problem with an obvious response. A chamber that is two degrees off, or holding twelve percent less humidity than it reports, produces a trial that looks completed and is quietly not comparable to the one before it.

Look for a gradient, not an average

Single-point measurement at the sensor position will confirm what the controller already believes. The useful question is whether conditions are uniform across the growing space. Position within the chamber becomes a hidden experimental variable the moment it is not.

Multi-point verification across shelves and across the chamber depth is how you find this.

Humidity and temperature are coupled

Relative humidity depends on temperature, so a temperature control problem presents as a humidity problem and vice versa. Diagnosing them separately leads to chasing the wrong system. Both need to be measured together before drawing conclusions.

Lighting affects thermal load

Lighting is a significant heat source inside the chamber. Degraded output means less heat as well as less light, which shifts the balance the refrigeration system was set up for. A chamber that has drifted since a lamp change is usually telling you about thermal load rather than about a control fault.

Check the programme is executing as written

Programmes get modified over years by different people. Before diagnosing hardware, confirm that what the controller is actually executing matches what the current protocol says it should be. This is a surprisingly frequent root cause and costs nothing to check.

Schedule maintenance between trials

Chamber maintenance carried out mid-trial is disruptive at best and invalidating at worst. Give your service provider your trial calendar and let the work fall into the gaps. It is a straightforward conversation that avoids a genuinely expensive problem.

Dealing with this right now? Call 431-336-4558 — answered at any hour by Dustin or Colleen.

Dustin Riddoch

Owner, DR LAB WORx Inc

Interprovincial Red Seal journeyperson in Refrigeration and Air Conditioning with over 20 years in laboratory refrigeration. Thermo Fisher Scientific third-party representative for Manitoba and Northwestern Ontario since 2007, factory trained and authorized on PHCbi product lines.

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